One of the advantages is that due to their structure and smaller size, antibody fragments possess properties (e.g., easier tissue penetration) that suit a range of diagnostic and therapeutic applications. This platform approach enables increased efficiency and productivity in developing therapeutics based on antibody fragments.Īntibody fragments (e.g., Fab, scFv, domain antibodies, etc.) are set to become the next important class of proteinbased biotherapeutics after monoclonal antibodies (MAbs). Using Capto™ L, Capto SP ImpRes, and Capto Q media in the three step process resulted in efficient removal of the main contaminants and high yields (≥ 87%) over the entire process. This general approach supports Quality by Design (QbD), where the plates give the characterized space and the DoE in columns render both the design and the control space. Design of Experiments (DoE) was used to further optimize the conditions for each step. Next, capacity studies were performed in small columns with purified protein, and elution studies in columns were performed to find the optimal elution pH. First, chromatography media (resins) were screened in 96-well PreDictor™ plates, using wide wash and elution conditions. Macro-Prep resin operate well at low and medium pressures and exhibit minimal volume changes in response to variations in pH or ionic strength.This Application note describes a three-step purification process of a Fab originating from an E. These rigid macroporous, hydrophilic 50 µm resin provide exceptional capacity, resolution, and throughput in a chemically and mechanically stable form. Macro-Prep ion exchange resin meet the demands of analytical, semipreparative, and process-scale applications. Nuvia S is a flexible alternative that may be used both as a capture or polishing solution. Nuvia S provides very high capture and exceptional flow properties designed to meet current and future process needs. Nuvia™ S is an ultra high-capacity, next-generation cation exchange resin built on the industry proven UNOsphere base matrix technology. These resin are designed for process-scale purification of a wide variety of therapeutic proteins. Nuvia cPrime hydrophobic cation exchange resin are a new addition to Bio-Rad's family of mixed-mode purification products. Nuvia™ cPrime™ Hydrophobic Cation Exchange Resin UNOsphere Rapid S support combines high binding capacity, fast mass transfer, excellent flow characteristics, and robust physical properties to make it an exceptional choice for process chromatography. The support has been optimized to deliver speed and throughput in capture and interresinte purification steps. UNOsphere Rapid S support is a strong cation exchange support for process chromatography. We are now offering Nuvia Q resin, our exciting new high capacity anion exchanger delivering excellent resolution capabilities of feedstream contaminants such as host cell proteins and double-stranded DNA. The resin are built on a robust base matrix with proprietary surface extender technology. Nuvia resin are a family of ultra high capacity ion exchangers designed for optimal performance in bioprocessing workflows.
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